Journal: Journal of Medical Signals and Sensors

Article Title: Evaluation of the Radiosensitizing Potency of Bromelain for Radiation Therapy of 4T1 Breast Cancer Cells

doi: 10.4103/jmss.JMSS_25_18

Figure Lengend Snippet: Bromelain sensitizes 4T1 cells to irradiation. Bromelain was administered 24 h before X-rays and 7 days later clonogenic assay was carried out. Control group cells (a). Cells were treated with Bromelain (b). 2 Gy (c). Bromelain + 2 Gy (d), 4 Gy (e). Bromelain + 4 Gy (f). 6 Gy (g). Bromelain + 6 Gy (h). The survival curves derived from the clonogenic assay experiments treated with or without bromelain were significantly different ( P < 0.05) (i). The experiments were performed in triplicate and data are presented as mean ± standard deviation of three separate experiments

Article Snippet: 4T1 (mouse BC) and L929 (mouse fibroblast) cell lines were obtained from the Pasteur Institute (Tehran, Iran).

Techniques: Irradiation, Clonogenic Assay, Control, Derivative Assay, Standard Deviation

Journal: Journal of Medical Signals and Sensors

Article Title: Evaluation of the Radiosensitizing Potency of Bromelain for Radiation Therapy of 4T1 Breast Cancer Cells

doi: 10.4103/jmss.JMSS_25_18

Figure Lengend Snippet: The effect of bromelain on apoptosis in 4T1 cells. The cells have been treated with bromelain and/or radiation, harvested 8 h after treatment, stained with Annexin V/PI and analyzed by flow cytometric analysis. Control group cells (a). Cells were treated with bromelain (b). 2 Gy (c). Bromelain + 2 Gy (d), 4 Gy (e). Bromelain + 4 Gy (f). 6 Gy (g). Bromelain + 6 Gy (h). The bar graph shows the percentage of all apoptotic events following bromelain and/or radiation treatment (i). The data are presented as mean ± standard deviation of at least three independent experiments

Article Snippet: 4T1 (mouse BC) and L929 (mouse fibroblast) cell lines were obtained from the Pasteur Institute (Tehran, Iran).

Techniques: Staining, Control, Standard Deviation

Journal: International Journal of Nanomedicine

Article Title: Breakthrough of Hypoxia Limitation by Tumor-Targeting Photothermal Therapy-Enhanced Radiation Therapy

doi: 10.2147/IJN.S450124

Figure Lengend Snippet: Multi-modality imaging by PIBD. ( A ) In vitro CT contrast images and CT values of PIBD at different concentrations; ( B ) In vitro PA contrast images and PA intensity of PIBD at different concentrations; ( C ) In vitro NIRF contrast images and NIRF intensity of PIBD at different concentrations; ( D ) In vivo PA images of tumors in 4T1 tumor-bearing mice after i.v. injection of PIBD at different time points; ( E ) Changes of PA-signal intensity within tumor regions at corresponding time points; ( F ) In vivo CT images of tumors in 4T1 tumor-bearing mice after i.v. injection of PIBD at different time points; ( G ) Changes of CT value within tumor regions at corresponding time points; ( H ) In vivo NIRF images of tumors in 4T1 tumor-bearing mice after i.v. injection of PIBD at different time points; ( I ) Changes of NIRF fluorescence intensity within tumor regions at corresponding time points; ( J ) In vivo NIRF images of tumor and organs in 4T1 tumor-bearing mice after i.v. injection of PIBD at 24h.

Article Snippet: 4T1 cells (Mouse breast cancer cells) was purchased from Wuhan Punosai Life Technology Co., LTD.

Techniques: Imaging, In Vitro, In Vivo, Injection, Fluorescence

Journal: International Journal of Nanomedicine

Article Title: Breakthrough of Hypoxia Limitation by Tumor-Targeting Photothermal Therapy-Enhanced Radiation Therapy

doi: 10.2147/IJN.S450124

Figure Lengend Snippet: In vitro treatment. ( A ) Cell viability of 4T1 cells in different treatments by CCK8 assay; ( B ) Fluorescence intensity of DCFH-DA by different treatments; ( C ) Enhanced ROS production by PIBD in 4T1 cells. Confocal images (green fluorescence indicates positive staining for ROS stained with DCFH-DA) by different treatments (scale bar=100 μm); ( D ) DNA double strand breakage by PIBD in 4T1 cells. Confocal images (green fluorescence indicates positive staining for DNA double strand breakage stained with γ-H2AX) by different treatments (scale bar=10 μm). ( E ) Fluorescence intensity of γ-H2AX by different treatments; ( F ) Apoptosis rate of 4T1 cells in different treatments; ( G ) Apoptosis of 4T1 cells in different treatments by flow cytometry. (**P<0.01, ***P<0.001).

Article Snippet: 4T1 cells (Mouse breast cancer cells) was purchased from Wuhan Punosai Life Technology Co., LTD.

Techniques: In Vitro, CCK-8 Assay, Fluorescence, Staining, Flow Cytometry

Journal: Biosensors

Article Title: Highly Sensitive Detection of Hydrogen Peroxide in Cancer Tissue Based on 3D Reduced Graphene Oxide–MXene–Multi-Walled Carbon Nanotubes Electrode

doi: 10.3390/bios14060261

Figure Lengend Snippet: Quantitative cell viability results by CCK−8 assay for ( A ) 4T1 and ( B ) MCF−7 cells incubated with the 3D rGO–Ti 3 C 2 –MWCNTs electrode for 0, 1, 2, 3, 4, and 5 h. ( C ) Reduction current response of the 3D rGO–Ti 3 C 2 –MWCNTs electrode with the addition of fMLP, catalase, and fMLP-free PBS to deoxygenated 0.01 M PBS solution containing 7.0 × 10 6 4T1 cells, 5.0 × 10 6 MCF−7 cells, and no cells. ( D ) Amperometric current response of the 3D rGO–Ti 3 C 2 –MWCNTs electrode after separate injections of fMLP, catalase, and fMLP-free PBS into breast cancer tissue immersed in the deoxygenated 0.01 M PBS solution.

Article Snippet: Human breast cancer cell line MCF−7 and mouse breast cancer cell line 4T1 were purchased from Servicebio Technology Co., Ltd. (Wuhan, China).

Techniques: CCK-8 Assay, Incubation

Journal: Scientific Reports

Article Title: Increase in fatty acids and flotillins upon resveratrol treatment of human breast cancer cells

doi: 10.1038/s41598-019-50416-5

Figure Lengend Snippet: Effect of resveratrol on the cell viability of MCF-7 and MDA-MB-231 cells. ( a ) 4T1, ( b ) MCF-7 and ( c ) MDA-MB-231 cells (2 × 10 5 ) were incubated in the presence of different concentrations (10 to 300 μM) of resveratrol, or 0.1% DMSO (control), for 24 h at 37 °C. They were then washed and maintained with MTT (0.5 mg/mL) for 3 h at 37 °C for formazan crystals formation and the associated optical density was analyzed at 570 and 650 nm. The results were expressed as mean ± SD, n = 3, *p < 0.05, **p < 0.01, ***p < 0.001 significantly different (p < 0.05) from the values corresponding to the control, using One-way ANOVA, followed by multiple comparison test Dunnett.

Article Snippet: The human mammary gland/breast epithelial cell lines MCF-7 and MDA-MB-231, and the mouse mammary gland/breast epithelial cell line 4T1 were obtained from American Type Culture Collection (ATCC® HTB-22TM, HTB-26TM and CLR-2539TM respectively).

Techniques: Incubation, Control, Comparison

Journal: Iranian Journal of Parasitology

Article Title: Anticancer Activity of Hydatid Cyst Fluid along with Antigen B on Tumors Induced by 4T1 Breast Cancer Cell in a BALB/c Mice Model

doi: 10.18502/ijpa.v17i2.9542

Figure Lengend Snippet: Schematic illustration of AgB and HCF injection and 4T1 implantation in BALB/c mice

Article Snippet: Mouse breast adenocarcinoma (4T1) cell line was purchased from the National Cell Bank of Iran (Pasteur Institute, Tehran, Iran) and cultured in RPMI-1640 medium (Gibco: AC16-012) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco:42F0375K), 2mM L-glutamine, 100 μg/ml of streptomycin (Gibco, USA) and 100 units/ml of penicillin.

Techniques: Injection

Journal: Iranian Journal of Parasitology

Article Title: Anticancer Activity of Hydatid Cyst Fluid along with Antigen B on Tumors Induced by 4T1 Breast Cancer Cell in a BALB/c Mice Model

doi: 10.18502/ijpa.v17i2.9542

Figure Lengend Snippet: The tumor volume (Mean ± SEM, mm 3 ) of mice in control groups and mice injected with different concentrations of alum-absorbed hydatid cyst fluid, and antigen B (100, 300, and 500 μg/ml) following determined days after challenge with 4T1 tumor cells

Article Snippet: Mouse breast adenocarcinoma (4T1) cell line was purchased from the National Cell Bank of Iran (Pasteur Institute, Tehran, Iran) and cultured in RPMI-1640 medium (Gibco: AC16-012) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco:42F0375K), 2mM L-glutamine, 100 μg/ml of streptomycin (Gibco, USA) and 100 units/ml of penicillin.

Techniques: Control, Injection

Journal: Iranian Journal of Parasitology

Article Title: Anticancer Activity of Hydatid Cyst Fluid along with Antigen B on Tumors Induced by 4T1 Breast Cancer Cell in a BALB/c Mice Model

doi: 10.18502/ijpa.v17i2.9542

Figure Lengend Snippet: Histological findings of breast cancer tissue (stained with H & E) induced by 4T1 cell line with a magnification of (×10) and (×40); ( A ) control group; ( B ) group injected with 100 μg/ml AgB and HCF; ( C ) group injected with 100 μg/ml HCF; ( D ) group injected with 300 μg/ml AgB; ( E ) group injected with 300 μg/ml HCF; ( F ) group injected with 500 μg/ml AgB; ( G ) group injected with 500 μg/ml HC

Article Snippet: Mouse breast adenocarcinoma (4T1) cell line was purchased from the National Cell Bank of Iran (Pasteur Institute, Tehran, Iran) and cultured in RPMI-1640 medium (Gibco: AC16-012) supplemented with 10% heat-inactivated fetal bovine serum (FBS) (Gibco:42F0375K), 2mM L-glutamine, 100 μg/ml of streptomycin (Gibco, USA) and 100 units/ml of penicillin.

Techniques: Staining, Control, Injection